Recombinant D. melanogaster Neurocalcin homolog(Nca)

Drosophila melanogaster

Nca

Nca; CG7641; Neurocalcin homolog; DrosNCa

2-190

Recoverin family

Calcium-binding protein that is essential for promoting night-time sleep and inhibiting nocturnal hyperactivity. During the night-time it promotes sleep by limiting synaptic output from arousal- and wake-promoting neurons within the C01- and A05- domains that include the mushroom body, and the antennal mechanosensory and motor center. Regulated by the circadian clock network and light-sensing circuits that include the CRY photoreceptor; it is likely that both of these internal and external cues are required to define the temporal window (the night-time) during which this protein functions. Inhibits the phosphorylation of rhodopsin in a calcium-dependent manner.

KEGG: Dmel_CG7641; STRING: 7227.FBpp0074671; UniGene: Dm.6656

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

GKQNSKLKP EVLEDLKQNT EFTDAEIQEW YKGFLKDCPS GHLSVEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFLCALS VTSRGKLEQK LKWAFSMYDL DGNGYISRQE MLEIVTAIYK MVGSVMKMPE DESTPEKRTD KIFRQMDRNK DGKLSLEEFI EGAKSDPSIV RLLQCDPQSH

Full Length of Mature Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.