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Drosophila Targeted RNAi Service

Targeted RNA interference (RNAi) is a powerful genetic tool used in Drosophila melanogaster research to investigate gene function and manipulate gene expression. RNAi allows researchers to selectively silence or knockdown the expression of specific genes, providing valuable insights into their roles in various biological processes. CD BioSciences specialize in offering cutting-edge services in the field of Drosophila research. Our Drosophila Targeted RNAi Service leverages the power of RNA interference (RNAi) to enable precise genetic manipulation and investigation of gene function in Drosophila melanogaster.

Introduction to Drosophila Targeted RNAi

The principle of targeted RNAi in Drosophila research involves introducing synthetic siRNAs into the cells of the Drosophila melanogaster to silence the expression of a specific gene. This is typically achieved by injecting siRNAs into early-stage Drosophila embryos or by using genetic engineering techniques to generate transgenic flies expressing short hairpin RNAs (shRNAs), which are processed into siRNAs within the fly's cells. The siRNAs bind to their target mRNA molecules, triggering their degradation by an enzyme called Dicer, leading to a reduction or complete loss of the corresponding gene's expression.

Fig.1 Inductive expression of double-stranded RNA (dsRNA) by the Gal4/UAS system.

Fig.1 Inductive expression of double-stranded RNA (dsRNA) by the Gal4/UAS system. (Yamamoto-Hino M., et al. 2013)

In contrast to S2 cells, delivering dsRNAs into the Drosophila organism poses a challenge. To induce RNAi in Drosophila, libraries of fly strains bearing transgenes for the expression of hairpin type RNAs have been developed. Given that RNAi targeting essential genes can result in lethality, it is necessary to carefully control the expression of hairpin RNAs to ensure the stability of transgenic flies. Thus, in Drosophila, the Gal4/UAS system, commonly used to control gene expression, allows for the expression of dsRNA in target cells as hairpin RNA.

Our Services

CD BioSciences offers precise and efficient Drosophila targeted RNAi services that can help you unlock the mysteries of gene function. Our customized service has the following benefits:

  • Customized RNAi Design
    Our experienced team of scientists will work closely with you to design custom RNAi constructs tailored to your specific research objectives. We utilize advanced algorithms and databases to identify target genes and design RNAi molecules with high specificity and efficiency.
  • Generation of RNAi Constructs
    Once the RNAi targets are identified, we employ cutting-edge molecular biology techniques to generate high-quality RNAi constructs. Our process ensures precise insertion of RNAi molecules into the Drosophila genome, enabling effective gene knockdown.
  • Validation and Phenotypic Analysis
    To ensure the success of your experiments, we provide comprehensive validation and phenotypic analysis of RNAi-mediated gene silencing. Our team utilizes a variety of molecular and imaging techniques to assess the efficiency of knockdown and evaluate resulting phenotypic changes.

Why Choose Us?

CD BioSciences specialize in providing exceptional services in the field of Drosophila research. By leveraging our Drosophila targeted RNAi service, researchers gain a powerful tool to unravel the complexities of gene function in Drosophila. Our customizable service, combined with high-quality RNAi constructs and efficient delivery methods, empowers researchers to conduct precise gene silencing experiments, paving the way for significant discoveries in the field of Drosophila genetics. Contact us to learn more about our Drosophila targeted RNAi service and how it can accelerate your research efforts.

Reference

  • Yamamoto-Hino M, Goto S. In vivo RNAi-based screens: studies in model organisms. Genes, 2013, 4(4): 646-665.

For research use only. Not intended for any clinical use.

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