Germ Free Drosophila Models Service

• Project consulting & design
• Preparation process
• Dechorionation 8 germ free breeding
• Medium culture & sequence validation

• Project delivery
• Other sterile models customization (optional)
• Authenticating colonization (optional)
• Microbial colonization (optional)

• Germ free Drosophila basic service - Embryo dechorionation, strict sterile rearing, antibiotic added for next three generation to remove endosymbiotic bacteria, such as Wolbachia and Spiroplasma, Drosophila intestinal sterility validation by gut culture, etc.
• Antibiotic treatment - Depending on the client's experiment, we can feed antibiotics to Drosophila at larval and adult stages for stage-specific sterile treatment. Different sizes of antibiotic mixtures such as kanamycin, ampicillin and doxycycline are available.
• Sequencing Validation Service (optional) - 16S rDNA or 16S ARDRA (Amplified Ribosomal DNA Restriction Analysis) Sequencing.
• Mono/poly colonization services (optional) - The mono-associated or poly-associated state of the germ free Drosophila fed with the microbe of interest, clients can send us or tell us the target microbe; verification by selective media, or FISH (Fluorescence In Situ Hybridization), DGGE, 16s rDNA, etc.
• High-throughput germ free models (optional) – Constructs of various specific sterile Drosophila models that can be used for scientific experiments on human diseases, drug discovery, metabolic mechanisms, innate immunity, neural network regulation, etc. 

In principle, we ship germ free Drosophila as larvae or adults. But different project may have different requires. Our management team will explain the shipping process and precautions specifically to you prior to shipment. Final vials are kept at the factory for two weeks as a backup.

• Perfect aseptic operation process and equipment - Complete Drosophila stock, and supporting aseptic and microscopic equipment.
• Professional technicians - Skilled in aseptic operation, Drosophila breeding, transgene technique, microbial culture and sequencing analysis.
• Standard environmental conditions - Regular cleaning and disinfection, temperature, humidity, and light in accordance with the norms for maintaining Drosophila populations.
• Robust service system - One-stop service available based on the company's mature transgenic, testing, and data analysis platforms.
• Fast and stable supply - Competitively priced services and large quantities of high-quality sterile fruit flies in the shortest possible time.

• Interactions between the in vivo microbiome and pathogenic bacteria
• Mechanisms of host innate immune regulation
• Effect of behavior, e.g., diet, on the microbial community in vivo
• Role of the gut-brain axis in development, physiological regulation, genetics, epigenetic modifications and disease