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Drosophila Carbohydrates Metabolism Analysis Service

The two main forms of circulating carbohydrates in Drosophila are glucose and trehalose (the disaccharide of glucose). Carbohydrate metabolism is essential for all life and is involved both in direct cellular energy supply (glucose) and in biosynthesis, with excess sugars being stored as glycogen. Drosophila including genetically modified human disease models have been increasingly used to study the regulation of carbohydrate metabolism. Qualitative and quantitative metabolic analysis of various glycans in Drosophila is therefore essential for the identification of carbohydrate gene regulatory networks and local regulation of signaling pathways, as well as providing new insights into diseases with imbalanced glycolytic metabolism such as diabetes and cancer.

CD BioSciences uses biochemical methods to efficiently and accurately determine the levels of trehalose, glucose and glycogen in Drosophila samples. In addition, we perform comprehensive glycan composition and structural analysis of different tissues, organs and developing states of Drosophila through our professional glycan release platform and high-throughput mass spectrometry assay platform to obtain high-quality glycome results to meet the different needs of our customers and accelerate your research on the regulation of Drosophila glucose metabolism.

Intracellular sugar-responsive gene regulatory network in Drosophila

Fig.1 Intracellular sugar-responsive gene regulatory network in Drosophila (Mattila et al, 2017)

Our Service

  • Trehalose and Glucose
    Both trehalose and glucose can be present in Drosophila in free form. The glucose transporter protein is bidirectional and most free glucose and trehalose are present in the circulating fluid, and we recommend extracting hemolymph for accurate quantification of free glucose and trehalose. Trehalose requires digestion by trehalose to produce glucose. The process of glucose hydrolysis is accompanied by the production of NADH. CD BioSciences offers several enzymatic protocols to detect the amount of NADH and characterize the amount of free glucose and free trehalose, respectively.
  • Glycogen
    Most carbohydrates in Drosophila are stored as glycogen, which is normally mobilized in large amounts during intense exercise and stress. Accurate characterization and quantification of stored sugars is therefore essential to characterize the sugar metabolic activity of Drosophila. CD BioSciences provides a variety of enzymatic protocols to detect NADH content and characterize glycogen content.
  • Glycomics Analysis
    CD BioSciences provides mass spectrometry and chromatography-based Drosophila glycomics analysis for exploring the dynamic processes of carbohydrate synthesis and dissociation during development. Our HPLC, LC-MS/MS detection platform analyzes the content and abundance of monosaccharides, disaccharides and polysaccharides in samples, including mannose, galactose, and fucose, etc. In addition, CD BioSciences has professional polysaccharide release platform and separation detection platform, UHPLC and MALDI-TOF MS/MS, supporting high throughput analysis of polysaccharides (e.g., N-Glycan, O-Glycan, and glycosphingolipid GSL) to help customers' in-depth study of Drosophila glycosylation.

General Workflow

General Workflow

Why CD BioSciences?

  • Rich experience in carbohydrates metabolism research of Drosophila
  • Excellent and professional research team and technical testing platform
  • Flexible Drosophila carbohydrates analysis service
  • Reliable and visual results with good repeatability
  • Fast turnaround, cost-effective and transparent pricing

CD BioSciences is a global biotechnology company enabling cutting-edge scientific research in Drosophila. We offer a one-stop Drosophila carbohydrates metabolism assay, including biochemical analysis and glycomics analysis. Our team of professionals provides 24 × 7 customer service to every client for saving time and effort. There are no limitations of our service. If you need any further information or have any question, please feel free to contact us.

Reference

  • Mattila J, et al. (2017). Regulation of carbohydrate energy metabolism in Drosophila melanogaster. Genetics. 207(4), 1231-1253.

For research use only. Not intended for any clinical use.

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