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Drosophila mRNA-Seq Service

As the messenger of DNA transcription, mRNA is an essential component of the transcriptome. It delivers genetic information to the ribosome and serves as a template to guide peptide chain synthesis. To uncover the mysteries of gene expression, CD BioSciences offers advanced mRNA-Seq services for Drosophila mRNA analysis.

Introduction to mRNA-Seq

RNA-Seq is a sequencing technique that uses next-generation sequencing to reveal the presence and quantity of RNA in a biological sample, representing an aggregated snapshot of the transcriptome. As the messenger of gene expression, although mRNA only accounts for about 2%, it is the center of transcriptomics research, containing the most genetic information. Therefore, mRNA-seq technology, which specializes in the detection of all mRNAs, has become a powerful tool for RNA research, as well as one of the most widely used experimental techniques in RNA qualitative and quantitative studies.

The process of gene expression. Fig. 1 The process of gene expression

Applications of mRNA-Seq

With the rapid development of technology, mRNA-Seq has become an indispensable tool for analyzing differential gene expression, mRNA variable shearing, and transcriptomics studies. mRNA-Seq technology has many applications include but are not limited to:

  • Differential gene expression analysis
  • Gene expression level analysis
  • Transcriptome structure analysis
  • New transcriptome discovery
  • Transcriptome variant analysis
Schematic of RNA-Seq in Drosophila. Fig. 2 Schematic of RNA-Seq in Drosophila (Mclaughlin C N, et al., 2021)

mRNA-Seq Applied to Drosophila Research

Transcriptome studies provide insights into gene expression, cellular regulation, and biomarkers, and are an integral part of numerous biological and pharmaceutical studies. Coincidentally, Drosophila is the classic model organism, making it an excellent choice for biological research. By studying the PER gene, mRNA and protein, scientists revealed the regulatory mechanism of circadian genes in Drosophila, for which they were awarded the Nobel Prize in 2017. mRNA-Seq technology is not only a powerful tool for transcriptome research, but also for combined genomics, proteomics and metabolomics research.

Our Services

With professional teams and top-notch sequencing platforms, CD BioSciences provides high-qulity mRNA-sequencing service, from sampling and library construction to sequencing.

Drosophila mRNA-Seq Service-3

Experimental design

With standardized experimental procedures and years of research experience, we offer custom mRNA-Seq experimental protocol design. If you already have a protocol, we can also help you improve it.

Sampling

There are appropriate sampling methods for different Drosophila tissue parts. Our technicians have rich experience in Drosophila sampling to ensure the highest quality of tissues extracted.

mRNA extraction

Reducing interference from other RNAs is very important for mRNA-Seq. We utilize the Poly (A) tail of mRNA to enrich mRNA, and our rich experience in this field allows us to extract mRNA with very high purity.

cDNA library construction

Constructing cDNA libraries to get high quality clean reads is the key to ensuring the accuracy of sequencing. We provide different library construction methods according to customers' needs. No matter whether forward, reverse or non-stranded, we can satisfy and guarantee its high quality.

Sequencing

After cDNA library construction, we help you complete mRNA sequencing on the Illumina Sequencing Platform. Whether single-end reads or paired-end reads, we strictly demand data quality.

Data analysis

The complexity of mRNA-Seq data may give you a headache. We have a dedicated bioinformatics analysis platform that enables fast and stable data analysis and delivery of results.

CD BioSciences brings a wealth of experience to the Drosophila research domain. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Leveraging our proprietary platform, we've successfully executed numerous Drosophila-based research projects. If you are interested, don't hesitate to contact us for more information and quotes.

Reference

  • Mclaughlin C N.; et al. Single-cell transcriptomes of developing and adult olfactory receptor neurons in Drosophila. eLife. 2021, 10: e63856

For research use only. Not intended for any clinical use.

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