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Site-Specific Recombination Systems in Drosophila

CD BioSciences as an experienced biological scientists' project management team, focuses on the critical decisions needed for cutting-edge Drosophila research. Our unique platform can meet customers' personalized research needs. Our service covers whole-genome insertion, excision, inversion and translocation by site-specific recombination systems. We will regularly follow up the progress and the next strategy with you during the project. Once completed, we send customers with final reports, including strategy design, raw data and quality analysis.

Site-Specific Recombination Systems in Drosophila Transgenesis

Site-specific recombination systems are powerful tools for manipulating genetic material in organisms like Drosophila. Three commonly used systems are the Flp-FRT system, Cre-loxP system, and PhiC31 integrase system.

  • The Flp-FRT system utilizes the Flp recombinase enzyme and Flp recognition target (FRT) sites. By expressing Flp in specific cells or tissues, targeted DNA sequences flanked by FRT sites can be inverted, excised, or rearranged.
  • The Cre-loxP system employs the Cre recombinase enzyme and loxP sites. Cre-mediated recombination allows for DNA excision, inversion, or translocation when loxP sites are present. This system facilitates tissue-specific gene activation or deletion.
  • The PhiC31 integrase system utilizes the PhiC31 integrase enzyme and attP/attB sites. By expressing PhiC31 integrase in Drosophila, it can mediate efficient DNA integration at specific attP sites within the genome using attB sites located on the incoming DNA.
Fig.1 Modifying genome structure and gene expression with site-specific recombinases.

Fig.1 Modifying genome structure and gene expression with site-specific recombinases. (Nern A., et al. 2011)

These site-specific recombination systems provide precise control over genetic modifications, enabling targeted gene expression, gene knockout, gene replacement, or transgene integration in Drosophila, thereby facilitating the study of gene function and development.

Our Services

Our expertise lies in utilizing site-specific recombination systems, including the Flp-FRT system, Cre-loxP system, and PhiC31 ntegrase system, to create precise genetic modifications in Drosophila models. With our services, you can achieve highly targeted and reliable transgenesis, enabling you to advance your research goals with confidence.

Flp-FRT System in Drosophila

Flp-FRT System in Drosophila

Our skilled team of experts can generate precise genetic modifications by using the Flp recombinase enzyme to target specific DNA sequences flanked by FRT sites.

Cre-loxP System in Drosophila

Cre-loxP System in Drosophila

With this system, our team can efficiently manipulate DNA sequences flanked by loxP sites using the Cre recombinase enzyme.

PhiC31 Integrase System in Drosophila

PhiC31 Integrase System in Drosophila

Our transgenesis services based on the PhiC31 integrase system enable you to achieve stable genomic integration of your transgenes into specific attP landing sites.

Why choose Us?

  • Experienced Team: Our team of experts has extensive experience in Drosophila research and genetic manipulation, ensuring accurate and reliable results.
  • Tailored Solutions: We understand that each research project is unique, and we work closely with you to design customized approaches that align with your specific objectives.
  • Cutting-edge Technology: We employ state-of-the-art techniques and equipment to ensure the highest quality results for your transgenic drosophila models.
  • Timely Delivery: We are committed to delivering your project on time, allowing you to progress swiftly with your research.

Want to Learn More?

CD BioSciences is committed to advancing Drosophila research by providing cutting-edge tools and technologies for genetic manipulation. Our experienced team leverages these site-specific recombination systems to provide tailored transgenesis services, ensuring precise and efficient genetic modifications in Drosophila. Contact us today to discuss your specific research needs and discover how our expertise can advance your studies.

Reference

  • Nern A, Pfeiffer B D, Svoboda K, et al. Multiple new site-specific recombinases for use in manipulating animal genomes. Proceedings of the National Academy of Sciences, 2011, 108(34): 14198-14203.

For research use only. Not intended for any clinical use.

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