Flp-FRT System in Drosophila
At CD BioSciences, we specialize in providing exceptional services in the field of Drosophila research. Our customized transgenesis services based on the Flp-FRT system offer a cutting-edge solution for genetic manipulation in Drosophila melanogaster. With our expertise and state-of-the-art facilities, we enable researchers to unlock new possibilities and gain valuable insights into genetic mechanisms.
Flp-FRT Systems in Drosophila Transgenesis
The Flp-FRT system is a powerful tool used in Drosophila transgenesis to manipulate and study genes of interest. The system consists of two key components: the Flp recombinase enzyme and the Flippase Recognition Target (FRT) DNA sequences. The Flp recombinase enzyme recognizes and binds to the FRT sequences, which are strategically placed in the genome or within transgenes. When the Flp enzyme binds to two FRT sites in an inverted orientation, it catalyzes a recombination event between them. This recombination event excises the DNA segment located between the FRT sites, resulting in the deletion or rearrangement of genetic material.
Fig.1 An example of FLP/FRT mediated loss-of-function clones. (Weasner BM., et al. 2017)
By strategically placing FRT sites around genes of interest, researchers can precisely control their expression or remove them altogether. This system enables the creation of genetic modifications, such as gene knockouts, conditional gene expression, or tissue-specific gene manipulation. The Flp-FRT system provides a versatile and precise method for studying gene function and understanding biological processes in Drosophila and has greatly contributed to the field of genetics and developmental biology.
We specialize in providing customized transgenesis services utilizing the Flp-FRT system. Our team of experienced scientists and researchers is dedicated to assisting you in your Drosophila research by offering tailored solutions that meet your specific needs. With our expertise in the Flp-FRT system, we can efficiently generate transgenic flies, enabling precise genetic manipulations and accelerating your research. Here are the key components of our service:
Consultation and Project Design
We begin by collaborating closely with you to understand your research objectives and experimental requirements.
Our team of experts will work with you to design a customized transgenesis strategy using the Flp-FRT system.
Vector Construction and Optimization
Once the project design is finalized, our scientists will proceed with constructing the required vectors for the Flp-FRT system.
Using advanced molecular biology techniques, we will engineer the necessary DNA constructs, including the gene of interest, selection markers, and Flp recognition target (FRT) sites.
Injection and Transgenic Drosophila Generation
Performing microinjections into Drosophila embryos with carefully designed DNA constructs. This step involves the precise delivery of the transgenesis constructs into the germ cells of the developing embryos.
Screening and Selection
Following the injection, we employ rigorous screening methods to identify the successfully transformed flies. This includes techniques such as PCR analysis, fluorescent marker detection, or other appropriate methods based on your project requirements.
Validation and Characterization
Our experts perform comprehensive analyses, including genotyping, expression profiling, and phenotypic evaluation, to validate the desired genetic modifications and confirm the functionality of the transgenic lines.
Deliverables and Reporting
Finally, we provide you with detailed documentation and a comprehensive report summarizing the results of the transgenesis service.
We also offer ongoing support and consultation to assist you in furthering your research.
Want to Learn More?
With our expertise in the Flp-FRT system, CD BioSciences offers a comprehensive workflow for customized transgenesis services. By leveraging this powerful genetic tool, we empower researchers to manipulate and study specific genes in Drosophila, enabling breakthrough discoveries and advancements in the field. Contact us to discuss how our transgenesis services can contribute to your research goals.
- Weasner B M, Zhu J, Kumar J P. FLPing genes on and off in Drosophila. Site-Specific Recombinases: Methods and Protocols, 2017: 195-209.
- Horn C, Handler A M. Site-specific genomic targeting in Drosophila. Proceedings of the National Academy of Sciences, 2005, 102(35): 12483-12488.
For research use only. Not intended for any clinical use.