Services

Inquiry

Drosophila Stable Isotope Tracer Analysis Service

Isotope tracer analysis, also called metabolic flow analysis, uses stable isotope labeled compounds by directly measuring downstream metabolic fluxes to infer the rate of turnover, direction and distribution pattern of the compound in the intracellular metabolic pathway. In Drosophila, detection and analysis of labeled intermediate metabolites can be performed with atomic level resolution by direct feeding of isotopically labeled compounds (lipids, proteins or amino acids, carbohydrates, etc.) for a defined period of time. This approach is currently used to analyze lipid synthesis, diet-induced obesity and glucose oxidation.

With a specialized stable isotope labeling platform, CD BioSciences offers a wide range of labeled compounds for Drosophila metabolic flow analysis (e.g., 2H, 13C and 15N). This includes sample processing of the target tissue or whole within a defined time frame, measurement of molecular mass or centrifugation techniques to differentiate isotopes, and analysis of isotope distribution using GC-MS, LC-MS or NMR to reveal important biogenesis mechanisms, targets of action, etc.

Basic principles of stable isotope tracer analysis - 13C-labeled glucose

Fig.1 Basic principles of stable isotope tracer analysis - 13C-labeled glucose (Li et al, 2017)

Our Service

CD BioSciences has developed a metabolic flow analysis platform for Drosophila that reveals the combined effects of gene expression, enzymatic reactions, and environment on the metabolism of specific compounds. Our CE-/GC-/LC-MS and NMR platforms are widely applicable for monitoring and analyzing metabolic kinetics at any stage or site in Drosophila. Our platform is built with stringent quality management controls. We provide custom readable results reports upon project completion to accelerate your research. Our services don't stop there.

  • Various Drosophila samples. Anatomical samples of head, crop, midgut, hindgut, malleoli, accessory glands, testes, ovaries and cuticle.
  • Various stable isotopes, 2H, 13C and 15N to label different compounds such as glucose, long chain fatty acids.
  • Flexible methods for metabolic flux analysis. Customized detection of small and highly polar molecules (e.g., pyruvate and lactate), large and hydrophobic molecules (ultra-long chain fatty acids, etc.) and molecules requiring special conditions (e.g., NADH) over feeding time.
  • Different metabolic flux analysis methods. Targeted analysis like glucose metabolic flux, glutamine flux, TCA cycle metabolic flux, and stoichiometric metabolic flux analysis to discover new metabolites.
  • Data Analysis. In-house data mining techniques are used to perform comprehensive and in-depth data analysis, including principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and volcano plot, etc.

Service Highlights

  • Quantitative characterization of dynamic changes in cellular metabolism and fine distribution of fluxes in metabolic pathways.
  • Accurate metabolite pool turnover information and source information
  • Accurate and efficient identification of targeted pathways, effectively revealing changes in metabolic pathways of interest
  • Rapid determination of the effect of external stimuli or genetic defects on metabolic system response
  • Targeted determination of dynamic metabolic change processes in Drosophila metabolizing dietary compounds.

General Workflow

Workflow of Drosophila stable isotope tracer analysis - CD BioSciences

Why CD BioSciences?

  • Rich experience in metabolic flux analysis of Drosophila
  • Excellent and professional research team and project management
  • Comprehensive separation and detection platform
  • Repeatable, custom-processed interpreted data
  • Fast turnaround, cost-effective and transparent pricing

CD BioSciences is a global biotechnology company enabling cutting-edge scientific research in Drosophila. We offer customized stable isotope tracer analysis and downstream metabolomics analysis. Our team of professionals provides 24 × 7 customer service to every client for saving time and effort. There are no limitations of our service. If you need any further information or have any question, please feel free to contact us.

References

  • Li H, et al. (2017). Methods for studying the metabolic basis of Drosophila development. Wiley Interdiscip Rev Dev Biol. 6(5):10.1002/wdev.280.
  • Cox JE, et al. (2017). Metabolomic studies in Drosophila. Genetics. 206(3), 1169-1185.

For research use only. Not intended for any clinical use.

Related Services