P-element Mediated Transgenesis Service
P-elements are mobile genetic elements found in the genome of Drosophila species. They were initially discovered in the 1970s and have since revolutionized the field of genetics and transgenesis. This transposition mechanism allows researchers to precisely introduce new genes or modify existing ones in Drosophila, enabling a wide range of genetic studies and functional analyses. CD BioSciences is a global research company focused on Drosophila scientific solutions. Based on our cutting-edge Drosophila transformation technology platform, we offer customized P-element mediated transgenic services to address technical challenges in the Drosophila transgenics field.
Mechanism and Regulation of P-element Transposition
P-elements encode an enzyme, a transposase that is responsible for mobilizing the P-element DNA. While the specifics of transposition vary among DNA transposon families, the general process involves six essential steps transposase-transposon DNA binding, the pairing of the transposon ends (synaptic complex formation), donor DNA cleavage, target DNA capture, strand transfer (integration) and disassembly/DNA repair.
Fig.1 Model of P-element transposition pathway. (Ghanim GE., et al. 2020)
Similar to other DNA transposons, the P-element transposase initially binds to specific sites on the transposon. Once it recognizes one transposon end, the P-element transposase, dependent on GTP, captures and pairs the second end, creating a paired-end complex. Sequential cleavage of each P-element end releases the transposon from the surrounding host genome, resulting in the formation of the cleaved donor complex.
After donor DNA cleavage, the excised transposon-transposase nucleoprotein complex captures a target DNA and integrates the P-element. The mechanisms of disassembly and DNA repair at the target site remain unknown. However, it is understood that the double-strand break generated at the donor site can be repaired through either the homologous recombination-dependent (HR) pathway or the non-homologous end joining (NHEJ) pathway of DNA repair.
Our Services
Our streamlined workflow for Drosophila P-element mediated transgenesis service is designed to provide you with reliable and reproducible results. The process involves the following key steps:
Consultation and Project Design
Our team of experienced scientists will work closely with you to understand your research goals and experimental requirements.
Offering personalized consultations to design a transgenesis strategy that best suits your needs.
Construct Design and Generation
Assisting you in designing the P-element-based transgenic constructs tailored to your specific genetic modifications.
Transgenesis and Embryo Injection
Using state-of-the-art techniques, we generate transgenic Drosophila strains by microinjecting the meticulously designed constructs into pre-blastoderm embryos.
Screening and Identification
After the injection, we employ advanced screening methodologies to identify successfully integrated transgenic lines.
Validation and Phenotypic Analysis
Offering comprehensive phenotypic analysis services to validate the successful integration of transgenes and assess the desired genetic modifications.
Our cutting-edge imaging technologies and molecular techniques ensure accurate characterization and interpretation of results.
In addition, we offer additional customization options, such as Drosophila balancing service, Drosophila drug screening service, or Drosophila gene editing service, to expand the versatility of your transgenic Drosophila lines.
Want to Learn More?
CD BioSciences is committed to being your primary partner in Drosophila research and innovative biotechnology services. We offer a 24 × 7 professional and customized technical services to every customer. Our P-element mediated transgenesis service is designed to revolutionize your research by providing efficient and reliable methods for genetic manipulation in Drosophila melanogaster. If you are interested in our services, please contact us for more information and a detailed quote.
References
- Sonane M, Goyal R, Chowdhuri D K, et al. Enhanced efficiency of P-element mediated transgenesis in Drosophila: Microinjection of DNA complexed with nanomaterial. Scientific Reports, 2013, 3(1): 3408.
- Ghanim G E, Rio D C, Teixeira F K. Mechanism and regulation of P element transposition. Open biology, 2020, 10(12): 200244.
For research use only. Not intended for any clinical use.
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