Drosophila Mitochondrial Genomics

Mitochondria are present in almost all cell types in animals, including humans. Cellular energy production depends on mitochondria for the production of ATP and metabolic by-products through oxidative phosphorylation (OXPHOS) and can be involved in a variety of life activities including respiratory chain energy supply, tissue development, apoptosis and innate immunity. Mitochondrial function depends on approximately 1200 - 1500 proteins, most of which are encoded by the nuclear genome and transported to the mitochondria, and therefore mitochondrial variation shows multiple patterns of inheritance.

Quantitative genetic and genomic approaches have been applied to identify the underlying nuclear genetic machinery, but studies on the function and variation of the maternally inherited mitochondrial genome are limited. Drosophila melanogaster is a powerful and genetically manageable model for studying mitochondrial genetics, and the Drosophila mitochondrial genome is conserved with that of humans.

CD BioSciences targets the cutting-edge research of mitochondrial genomics. Not only do we study the structural, functional and genetic characteristics of mitochondria through Drosophila, but we also use Drosophila genetic mutants to help our clients discover the mechanisms of multi-system dysfunctional diseases caused by mitochondrial variants, including neurodegenerative diseases (Parkinson, Alzheimer and Huntington disease), cardiovascular diseases, cancer and the aging process. CD BioSciences offers a total solution for mitochondrial genome (Mt genome) sequencing and analysis through Illumina and PacBio platforms.

Fig.1 Studying mitochondrial function and energy production in Drosophila (Salminen et al. 2020)

What Can We Offer?

• Mt Genome Sequencing
• Mt genome full-length sequencing.
• Supporting Drosophila Mt genome function-deficient mutants and other insect species.
• Detection of preference codon usage and nucleotide composition.
• Identification of rare mutations in the MT genome.
• Detection of epigenetic modifications.
• Mt Exome Sequencing & Targeted Sequencing
• Whole-exome sequencing analyzes the protein-coding regions of the genome, with the addition of untranslated regions (UTRs) and microRNAs if required.
• Targeted sequencing focuses on specific genes or targeted gene regions with known or suspected disease associations.
• Mitochondrial genome data analysis
• MtDNA copy number, tandem repeat sequences and genome rearrangements
• MtDNA single nucleotide mutation detection and annotation
• MtDNA heterogeneity detection and annotation
• MtDNA phylogenetic and evolutionary analysis

If you require Drosophila models carrying mitochondrial mutations and co-evolved experimental Drosophila, please contact our technical department online for more information.

General Strategy

Service Features

• Direct capture of MtDNA, accurate detection of mitochondrial genome, reducing the impact of nuclear genome.
• Powerful sequencing platform greatly improves the accuracy of variant detection and heterogeneity detection.
• 100% coverage of Mt genome, to obtain the full-length sequence.
• MtDNA is smaller than nuclear genome, greatly reducing sequencing costs.

Applications

• Study of the relationship between MtDNA and the nuclear genome.
• Study of mitochondrial uniparental inheritance mechanisms and mutations.
• To study the origin and evolution of mitochondria.
• To study the function of mitochondria in innate immunity, developmental aging, and disease mechanisms.

Why Choose us?

• Specialized sequencing platform - supports large scale computing, allowing simultaneous genome-wide multiple sequence alignment analysis from multiple species.
• Dedicated scientific team - one-to-one experiment planning to ensure scientifically meticulous results.
• Flexible integral service - reliable data and visual analytics to meet our clients' project needs.
• Fast and cost-effective supply - 7*24 service, close to customer's purpose, shorten customer's research time.

CD BioSciences addresses full-length sequencing technologies with an expanding database of mitochondrial genes. We provide each client with personalized analysis reports that elucidate the linkage between genotype and phenotype, genomic variant locus analysis and in-depth prediction. Our services do not stop there. If you are interested in or have any question, please feel free to contact us.

For research use only. Not intended for any clinical use.

Related Services

• Drosophila Chromosomal Dynamics
• Drosophila Whole Exome Sequencing
• Drosophila Whole Genome Sequencing