PiggyBac Transposon Mediated Transgenesis Service
PiggyBac transposon-mediated transgenesis is a powerful technique widely used in the field of genetics and developmental biology. It enables precise and efficient integration of DNA constructs into the Drosophila genome, allowing for targeted gene expression, functional studies, and genetic manipulation. At CD BioSciences, we are dedicated to advancing the field of Drosophila research and providing high-quality services to our customers. With our expertise and state-of-the-art facilities, we offer exceptional solutions for your transgenesis needs. Our experienced team of scientists is proficient in utilizing this technique to help you achieve your research goals.
Mechanism of PiggyBac Transposition
The PiggyBac transposon is a DNA element that can move or transpose itself within a genome. The transposition mechanism of PiggyBac involves two main components: the transposase enzyme and the inverted terminal repeats (ITRs). The ITRs are short DNA sequences located at each end of the transposon. The transposition process starts when the PiggyBac transposase recognizes and binds to the ITRs. The transposase cuts the DNA at the ITRs, creating sticky ends. Then, it forms a complex with the excised transposon and catalyzes the insertion of the transposon into a new genomic location.
Fig.1 The chemical steps of T. ni piggyBac transposition. (Yusa K. 2015)
PiggyBac is unique because it can transpose with high efficiency and precision in a wide range of organisms, including both vertebrates and invertebrates. It can integrate into the host genome at specific target sites or randomly, depending on the context. The ability to precisely insert and excise DNA sequences makes PiggyBac a valuable tool in genetic engineering and gene therapy research. Its versatile transposition mechanism has enabled applications such as gene delivery, mutagenesis, and the creation of transgenic organisms.
Our Services
Our streamlined workflow for Drosophila PiggyBac transposon mediated transgenesis service is designed to provide you with reliable and reproducible results. The process involves the following key steps:
Consultation and Experimental Design
Our team of experienced scientists will work closely with you to design a customized experimental strategy, ensuring that the PiggyBac transposon mediated transgenesis approach is tailored to your research objectives.
Construct Generation
This involves the design and assembly of the DNA fragments containing the gene(s) of interest, along with appropriate regulatory elements, promoters, and other genetic components.
Our team will employ advanced molecular biology techniques to construct the PiggyBac transposon vectors.
Drosophila Embryo Microinjection
Our highly skilled technicians will utilize precise microinjection techniques to deliver the constructs into the pronucleus of fertilized embryos.
Screening and Identification
After the injection, we employ advanced screening methodologies to identify successfully integrated transgenic lines.
Validation and Phenotypic Analysis
Offering comprehensive phenotypic analysis services to validate the successful integration of transgenes and assess the desired genetic modifications.
Ongoing Support and Customization
Throughout the entire process and beyond, our dedicated team will provide ongoing support and guidance.
Offering additional customization options, such as Drosophila balancing service, Drosophila drug screening service, or Drosophila gene editing service, to expand the versatility of your transgenic Drosophila lines.
Want to Learn More?
CD BioSciences is committed to being your primary partner in Drosophila research and innovative biotechnology services. By leveraging our Drosophila PiggyBac transposon-mediated transgenesis service, you can accelerate your research by efficiently generating stable transgenic lines for functional genomic studies, promoter analysis, and more. Our commitment to excellence ensures that you receive reliable and reproducible results to advance your scientific investigations. Contact us today to learn more about our service and how we can assist you in your research endeavors.
References
- Yusa K. piggyBac transposony. Mobile DNA III, 2015: 873-890.
- Sandoval-Villegas N, Nurieva W, Amberger M, et al. Contemporary transposon tools: a review and guide through mechanisms and applications of sleeping beauty, piggyBac and Tol2 for genome engineering. International journal of molecular sciences, 2021, 22(10): 5084.
For research use only. Not intended for any clinical use.
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