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Drosophila Comparative Genomics

Drosophila melanogaster genome contains about 14,000 protein-coding genes and a wide variety of non-coding loci, including 127 miRNA genes and multiple transposable elements. As a model organism, D. melanogaster has similar development, behavior and age to higher eukaryotes. Therefore, its genome is an important repository of information and reference for conducting comparative genomics studies, not only to reveal the conserved developmental signaling pathways, bioregulatory processes, and the genetic basis of species phylogeny and habitat adaptation in other arthropods, but also as a solid foundation and useful reference for studying the molecular mechanisms and functions of human diseases.

The large amount of data generated by comparative genomics requires powerful bioinformatics tools to systematically analyze and extract relevant information. CD BioSciences establishes an in-house computer development platform, developed multiple computing methods to avoid false positive predictions, and provides D. melanogaster genome-based comparative genomics services covering intergeneric comparisons, other non-Drosophila insects (mosquitoes, domestic silkworms, bees, etc.), organelle and cytoplasmic genomic comparisons, etc.

CD BioSciences uses multiple methods, e.g., PAML, PHAST, PILER, and HDMKPRF, etc., which allow genomic analysis of multiple species on a genome-wide scale at the same time. We provide reports on genome structure, chromosome organization, epigenetics, regulatory factors, and other results analysis.

Comparative genomics presupposes the availability of high-quality transcriptome data or gene mapping to reduce false positives and bias, you are appreciated to check our whole genome sequencing services for genomic sequencing and splicing solutions.

Our Service

• Comparisons with Drosophila subgroups
  The Drosophila contains about 2,000 species with distinct morphological, ecological, and behavioral differences. By multiple alignment of genomes within D. melanogaster, we can infer general principles of species formation, adaptation, and eventual evolution. This service aims to provide a more comprehensive understanding of gene structure and evolution, non-coding RNA conservation, and chromosome dynamics over a range of phylogenetic distances. Our analytical services can also assess the rate of evolution of coding genes and reveal the genetic basis of environmental adaptation.

• Comparisons with non-Drosophila insects
  CD BioSciences uses D. melanogaster's explicit phylogenetic knowledge to better help clients explore the development and evolution of other arthropods (Mosquitoes, wasps, beetles, etc.). Comparative genomics allows the annotation of coding and non-coding genes involved in biological processes such as conserved development (germline development, neurogenesis and sex determination), tissue differentiation (salivary glands, olfactory system), and behavioral regulation (parasitism, feeding) miRNA regulation.

• Comparative genomics of organelles or cytoplasm
  As the study progresses, we provide comparative genomic analysis based on known organelles and cytoplasm of Drosophila to resolve the regulatory expression mechanisms and dynamic evolution of mitochondria, lysosomes, cytoplasmic incompatibility induced by the endosymbiont Wolbachia, etc.

General Strategy

Service Features

• Two computational approaches, suitable for different research projects. De novo based approach, which predicts gene structure based only on a given DNA sequence; homology approach, which uses additional information from evolutionarily related genomes.
• Wide range of analysis, not only for whole-genome sequence alignment, but also for identification of protein-coding genes, exon-intron structures, non-coding RNA and regulatory elements.
• Comparative genomics allows dynamic detection and analysis of transposable element sequences. We support ab initio read, and unbiased prediction.
• From genome extraction and quality testing to library construction and computer sequencing, the experimental process undergoes strict quality control to ensure high accuracy of data.
• The standard automated preparation process effectively reduces costs and achieves high-cost performance.

Applications

• Uncovering the dynamic evolution of cellular substructures, and habitat adaptation among species.
• Studying the inherently repetitive nature and abundance of sequences such as transposons and satellite DNA in the genomes of plants and animals.
• Studying the evolution of de novo genes (Emergence from non-coding DNA).
• Determining the link between genotype and phenotype, including physiological characteristics of insects such as immune system, metabolic detoxification, parasitism and feeding.

Why Choose us?

• Specialized technology platform - supports large scale computing, allowing simultaneous genome-wide multiple sequence alignment analysis from multiple species
• Dedicated scientific team - enables versatile Drosophila cell modeling as a genetic and biochemical model system
• Flexible Integral Services - reliable data and visual analytics to meet our clients' project needs
• Fast and cost-effective supply - 7*24 service, close to customer's purpose, shorten customer's research time

CD BioSciences specializes in comparative genomics analysis. Using our Drosophila research platform, we provide our clients with a high-quality bioinformatic platform and final reports. Our 24-hour online service is always available for technical support. Our services do not stop there. If you are interested in or have any question, please feel free to contact us.

For research use only. Not intended for any clinical use.

Related Services

• Drosophila Genome-Wide Association Study