Drosophila miRNA Detection and Profiling

miRNA is a class of elements that regulate gene expression. miRNAs are participants in life activities as well as potential biomarkers. miRNAs can be present in Drosophila in clusters and are often co-expressed. Post-transcriptional processing of miRNA exists in Drosophila, and some introns can also be involved in regulation (mirtrons). Therefore, efficient capture and characterization of miRNA is a key step to study its targets and functions. Our Drosophila miRNA characterization service not only identifies novel miRNA, but also reveals the biogenesis and modification process of miRNA.

CD BioSciences, as an advanced biotechnology company, has established a professional laboratory platform for miRNA discovery, biogenesis analysis, and expression profiling in Drosophila and other species. We specialize in providing high quality and high coverage customized miRNA detection and analysis services to customers worldwide. We are committed to rapidly advancing our clients' research in life sciences and biomedicine, supporting a one-stop overall service.

Fig.1 Methods for profiling miRNAs in Drosophila (Chawla et al, 2016)

Our Service

To address the difficulty and complexity of miRNA characterization in Drosophila, we provide a full range of services from design and development to a variety of biochemical, immunological and sequencing analyses to help clients study transcription, processing and cellular level expression of miRNA in Drosophila. We have built an advanced and efficient technology platform to ensure the accuracy and repeatability of the results delivered to our clients, and to meet their various research purposes and applications.

Service	Technical Highlights	Descriptions
qPCR	Used for all miRNA detection, and not affected by genomic DNA contamination. High sensitivity and high throughput at single nucleotide levels. Absolute quantification.	Universal and specific assays based on sequence characteristics. Furthest capture of short sequences and processed miRNAs.
Northern Blot	Only detect known miRNAs. Low sensitivity.	Providing all kinds of probe synthesis service. Supporting all kinds of tissues and cell lines.
in situ Hybrization	Observed at cell resolution to monitor the dynamic expression of pri-miRNAs. Tissue-specific visualization.	Probe synthesis, including labeled locked nucleic acid (LNA) probes, which reduce the limitation of mature miRNA detection in Drosophila.
MiRNA Microarrays	Specific high-throughput analysis of miRNAs with known sequences was performed Used to detect miRNA expression in complex tissues or structures, e.g., fruit fly brains and embryos.	Sample processing. Purification of small RNAs. Fluorescence labeling. enrichment. Data standardization.
Next Generation Sequencing	High-throughput. Novel miRNAs discovery. Panoramic map acquisition of miRNA expression in various periods and tissues.	Data cleaning and annotation. Identification of differentially expressed miRNAs. MiRNAs copy number comparison, length distribution. Novel miRNA prediction. MiRNA structure analysis. Pre-miRNA cluster localization and analysis. Target gene prediction and functional analysis of miRNAs. MiRNA biogenesis prediction.
Transcriptional Reporters	Traceable miRNA expression patterns. Supporting Drosophila reporting systems in vivo and in cells.	The expression pattern of primary miRNA transcripts is determined by the expression of reporter genes (GFP, LacZ).
MiRNA sensors	Dynamic miRNA expression can be detected in living models. Limited by the flexibility and sensitivity of reporter genes.	Construction of tissue-expressed reporter genes containing one or more perfectly complementary miRNA binding sites in 3'UTR. The expression of reporter gene is observed.

Why CD BioSciences?

Rich experience in Drosophila miRNA research
Excellent and professional research team and technical platform
Flexible miRNA assay and bioprocess integration services
Reliable documented data, results and protocols
Fast turnaround, cost-effective and transparent pricing

CD BioSciences is an expert of miRNAs in fruit fly field. Our team of professionals provides 24 × 7 customer service to every client for saving time and effort. There are no limitations of our service. If you need any further information or have any question, please feel free to contact us.

Reference

Chawla G, et al. (2016). Analysis of MicroRNA function in Drosophila. Drosophila. 79-94.

For research use only. Not intended for any clinical use.

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