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Drosophila Lipid Metabolism Analysis Service

Lipids in Drosophila are abundant in terms of content and variety. Under normal conditions, dietary lipids are broken down in the gut into free fatty acids, monoacylglycerols and free sterols. They are then absorbed and transported throughout the body. Stored lipids are mainly in the form of diacylglycerol (DAG) or triglyceride (TAG), but Drosophila also contains various saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids and cholesterol. Lipids are not only energy sources for living organisms, but also involved in the synthesis and breakdown of substances. Lipid metabolism is an important physiological and biochemical phenotype that corroborates the metabolic physiological activity of Drosophila.

CD BioSciences uses biochemical methods for efficient and accurate determination of specific lipid contents (DAG or TAG) in samples. In addition, we detect and analyze a variety of lipids by GC-MS and LC-MS to obtain a high quality lipidome, which is essential to identify key regulators of lipid metabolism in Drosophila and reveal the important functions of lipid metabolism in development and disease.

Examples of the lipidome coverage for human and Drosophila melanogaster

Fig.1 Examples of the lipidome coverage for human and Drosophila melanogaster (Lange et al, 2019)

Our Service

  • Lipid Qualitative Analysis
    Drosophila mutation and clone analysis are widely used in qualitative analysis of neutral lipids in tissues and cells. CD BioSciences offers a number of lipids staining, such as Oil Red O, Sudan Black and Nile Red. Qualitative experiments will provide you with high-definition picture results.
  • TAG Quantitative Analysis
    TAG is the main lipid component of Drosophila and can preliminarily characterize lipid content and metabolic physiological activities. CD BioSciences uses lipoprotein lipase to cleave TAG, then the level of free glycerol is detected by colorimetric quantification and thin-layer chromatography (TLC).
  • Cholesterol Quantitative Analysis
    In addition to free cholesterol, Drosophila stores long-chain fatty acid esterified cholesterol in lipid droplets. Cholesterol is an essential component of cell membranes and a precursor for steroid hormone biosynthesis. CD BioSciences quantifies free cholesterol and cholersteryl ester by fluorometric assay.
  • Lipidomics Analysis
    CD BioSciences provides mass spectrometry-based semi-quantitative lipidomics analysis of Drosophila lipids for probing the kinetics of lipid remodeling during development, including embryo, larvae, pupae and adults. Our assay platform includes LLE, SPE, HPLC, LC-MS/MS and more, providing comprehensive coverage of over 400 lipid species, including glycerophospholipids, sphingolipids, triglycerides and sterols. In addition, CD BioSciences assay platform supports different samples such as hemolymph, as well as different types of tissues and organs (e.g., intestine, brain, and fat body) to meet the needs of our customers.

CD BioSciences is always committed to accelerating the speed of research projects and reducing the time and energy of customers. Our professional research team provides a one-stop service for Drosophila lipid analysis, including sample preparation, standard analysis and data processing.

If you need to mail samples, keep them with dry ice, not liquid nitrogen. If you need to test for rare lipids, please provide a standard sample.

General Workflow

General Workflow of the lipidome coverage for human and Drosophila melanogaster

Why CD BioSciences?

  • Rich experience in lipid metabonomics analysis of Drosophila
  • Excellent and professional research team and technical testing platform
  • Flexible Drosophila lipid analysis service
  • Reliable and visual results with good repeatability
  • Fast turnaround, cost-effective and transparent pricing

CD BioSciences is a global biotechnology company enabling cutting-edge scientific research in Drosophila. We offer a one-stop Drosophila lipid assay service, including biochemical analysis, imaging analysis and lipidomics analysis. Our team of professionals provides 24 × 7 customer service to every client for saving time and effort. There are no limitations of our service. If you need any further information or have any question, please feel free to contact us.

Reference

  • Lange M, et al. (2019). Liquid chromatography techniques in lipidomics research. Chromatographia. 82(1), 77-100.

For research use only. Not intended for any clinical use.

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