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Drosophila Neurons Calcium Imaging Service

Calcium imaging is a method of monitoring the concentration of calcium ions in tissues or cells using calcium ion indicators. In the resting state, most neurons have an intracellular calcium concentration of 50-100 nM, while when the neuron is active, the intracellular calcium concentration can rise 10-100 times, and calcium ions are the intracellular messengers for the cytosolic release process of synaptic vesicles. Therefore, calcium imaging techniques are often used in experiments to study the nervous system of Drosophila.

CD BioSciences uses confocal and two-photon microscopy to ensure that changes in calcium ions can be accurately detected in vivo or in vitro samples. Our Drosophila calcium imaging platform enables simultaneous monitoring of calcium changes within hundreds of neurons, identifying key neurons in the neural circuit, speeding up client research and saving effort and time.

Calcium Indicators

Fig.1 Calcium Indicators (Grienberger et al. 2012)

Our Service

CD BioSciences provides chemical and genetically encoded calcium indicators to support both in vitro and in vivo imaging. Our dedicated team provides total solutions for Drosophila calcium imaging to customers worldwide.

The principle of neuronal calcium imaging is to monitor neuronal activity by using special fluorescent dyes or protein fluorescent probes (calcium indicator) to express the calcium concentration in neurons through fluorescence intensity, by means of a strict correspondence between calcium concentration and neuronal activity.

  • Chemical Calcium Indicators
    Small molecules that specifically chelate calcium ions are used to emit light under a light source of also fixed wavelength. After chelating calcium ions, the wavelength of the excitation light source will change due to structural changes. Chemical calcium indicators can be used simultaneously due to their different affinities.
Chemical Calcium Indicator
  • Oregon Green-1
  • Fura-2
  • Indo-1
  • Fluo-3 / Fluo-4
Advantages
  • Fluorescence increased with the increase of intracellular calcium ions
  • Quantification of calcium
  • Good SNR and fast dynamics
Applications

Single cells or small groups of cells
  • Genetically Encoded Calcium Indicators (GECI)
    Commonly used for in vivo imaging. The more commonly used in Drosophila is the single-fluorophore GECIs. It is formed using green fluorescent protein (GFP) and its variants (e.g., cyclically arranged GFP, YFP, CFP), fused to calmodulin (CaM) and myosin light chain kinase M13 domains.
Genetically Encoded Calcium Indicator (GECI)
  • Camgaroo 1 / Camgaroo 2
  • GCaMP3 / GCaMP6
  • TN-XL / TN-XXL
Advantages
  • Suitable for in vivo imaging
  • Long-term effect
Applications

Molecular definitions of cell types and subcellular compartments

Why CD BioSciences?

Why choose us in Drosophila Neurons Calcium Imaging - CD BioSciences

CD BioSciences is an expert in Drosophila. We provide professional solutions for Drosophila neurons calcium imaging both in vivo and in vitro. Our team of professionals provides 24 × 7 customer service to every client for saving time and effort. There are no limitations of our service. If you need any further information or have any question, please feel free to contact us.

Reference

  • Grienberger C, et al. (2012). Imaging calcium in neurons. Neuron. 73(5), 862-885.

For research use only. Not intended for any clinical use.

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