CRISPR Oligonucleotides Design and Synthesis
The readily programmable CRISPR/Cas9 technologies have been transforming Drosophila genome engineering. The mature CRIPSR/Cas9 adaptive immune system has been simplified to two components for use in genome engineering: Cas9 endonuclease and a single chimeric RNA referred to as a chiRNA or single guide RNA (sgRNA). As CRISPR technology continues to improve, donor DNA is synthetized as homologous oligos integrated into any interested site of DNA for insertion, removal and substitution, relying on homologous recombination (HR) repair process.
CD BioSciences oligos synthesis platform equips state-of-the-art synthesizers and leads scientific teams experienced in the oligos synthesis as well as modification technologies. This allows us to provide high-quality CRISPR-related oligos products to scientific researchers and industrial customers around the world. In addition, we also maintain Drosophila high-throughput oligos pool to customers. Tailor-made approaches are always competent offered to accomplish every request in oligo synthesis.
CRISPR oligonucleotides design and synthesis platform
CD BioSciences offers a professional CRISPR oligonucleotides service including design, cloning, validation, to bring you pre-microinjection oligonucleotides or vectors. When you choose us as your trusted supplier, you maximize your budget and resources with our expertise in designing and synthesizing complex oligonucleotides, including sgRNA, single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and designed vectors. We additionally offer both satisfactory HPLC and PAGE purification for all oligos as a default option. MALDI-TOF is used for radical quality control of all oligos. We provide quality report along with product delivery.
The only thing you need offer is the NCBI gene ID or gene symbol of your interest gene and the type of mutation.
Our genomic engineering design experts establish a step-by-step custom CRISPR/Cas9 gene editing strategy as your requests.
- Sites design
Select target sites and synthesize oligos (sgRNA and donor DNA).
Construct optimal vector. We can transform plasmid provided from customers.
Screening and validation via PCR and sequencing.
2-5 μg lyophilized high-quality plasmids containing sequence verified reports and sequence alignment data ensuring the specificity will shipped to you in time.
Our CRISPR oligonucleotides design and synthesis service include
sgRNA is a component of CRISPR/Cas9 system, matching any desired sequence in the DNA and taking Cas9 protein to the target site.
Donor DNA can efficiently integrate longer sequences into target sites like tag and reporter or delete genes.
Why CD BioSciences?
- Record-proven expertise and experience in genetic engineering.
- Comprehensive targeting vector bank.
- Tested Gene synthesis and purification capabilities.
- Superior efficiency and fast turnaround in vector construction and validation.
CD BioSciences has years of experience spent in knock-out and knock-in vector construction and oligo synthesis. We have provided thousands of targeting vectors to customers worldwide. These have been used successfully in traditional and conditional genetic modification projects. 24x7 customer services will be provided for every client. Please feel free to contact us whenever you have any business and technical questions and requirements. We are looking forward hearing from you!
For research use only. Not intended for any clinical use.