Drosophila Transgenic Q-system Models Service
The Q-system is also a popular binary system in Drosophila allowing flexible manipulation of cellular and neural circuits, whose core component derived from the bread fungus Neurospora crassa that. The Q-system consists of two core components, a "driver" QF, the transcriptional activator, and a "reporter" element including QUAS (QF binding site) and downstream transgenic sequences encoding toxins, fluorescent proteins, ion channels or other effectors. In addition, two additional components are included, a "deterrent" QS and an anti-deterrent quinic acid QA. The Q-system is similar in principle to Gal4/UAS and LexA/LexAop. Since those systems do not interfere with each other, they can be combined to achieve complex and precise gene manipulation.
CD BioSciences offers a wide range of Drosophila transgenic Q-system models customization. We support various QF variants, including high level expression activator QF2 and QF2W, and complex activator Gal4QF and LexAQF, etc. As well as different copy number QUAS elements. CD BioSciences is committed to providing the most rapid and highest quality one-stop Drosophila model building service to our customers worldwide.
Fig.1 The Q-system in Drosophila (Potter et al. 2011, Riabinina et al. 2016)
Our Service
- General Strategy
CD BioSciences offers several variants of the Q-system for both basic gene regulation and complex genetic manipulation applications, such as classical MARCM, coupled MARCM and independent double MARCM strategies. The organic combination of the Q-system with the Gal4/UAS system enables overlapping cross-labeling of cellular subpopulations and more stringent and precise regulatory expression patterns, etc.
CD BioSciences provides one-to-one customization of the client's overall strategy, including experimental design, transgenic manipulation, quality control, complete report and Drosophila modeling, etc.
- Construction of QF Transgenic Drosophila
CD BioSciences supports both conventionally expressed and high-level expression QF constructs to meet the different needs of our customers. Our powerful Drosophila transgenic technology platform offers a comprehensive strategy of codon optimization, gene promoter cloning, enhancer traps, etc., with the ability to insert large fragments (20kb or 80kb).
- Construction of QS Effector Drosophila
QS is used to restrict the expression of reporter genes. CD BioSciences provides conditional and ubiquitous QS expression in transgenic Drosophila. The effect of QA depends on the amount and duration of feeding, and the expression pattern of target genes can be artificially regulated by modulating the concentration of QA.
- Downstream Detection Service
CD BioSciences, an expert in Drosophila research, offers comprehensive analysis methods for in vivo utilization of Q-system Drosophila, including neuron and circuit detection, identification of gene expression and knockout, mosaic analysis, etc. If you need or you are interested, please feel free to contact our online technicians.
Applications
- Labeling of tissues or different cell groups
- Target gene ectopic expression or knockout
- Cell ablation
- Neuron function and identification
- Mosaic analysis
At CD BioSciences, we draw upon our passions and the talents of scientists across a range of scientific disciplines to deliver tailored services and products for clients in need. We offer a comprehensive and scalable Drosophila Q-system mutant construction service. Our scientist team provides consistent results and superior efficiency improving fast turnaround answers and after-sale service. Contact us to learn more about how we support biotech laboratories and organizations in this fields.
References
- Potter CJ, et al. (2011). Using the Q system in Drosophila melanogaster. Nature protocols. 6(8), 1105-1120.
- Riabinina O, et al. (2016). The Q-system: a versatile expression system for Drosophila. Drosophila. 53-78.
For research use only. Not intended for any clinical use.
Related Services