Single-Cell RNA Sequencing in Drosophila
Single-cell RNA sequencing (scRNA-seq) has become a powerful tool to study cellular state and function at the single-cell level, revealing complex cell populations (e.g. Drosophila neuronal cells and fat body cells), gene regulatory relationships and cell developmental trajectories. The application of scRNA-seq to various Drosophila tissues and organs has dramatically changed transcriptomic studies in many life science research areas, such as Neurobiology, Immunology and Developmental Biology. However, scRNA-seq has extremely high technical requirements for single cell capture and library construction. Also, data processing is time consuming, which is a speed limiting part of the project.
CD BioSciences provides one-stop Drosophila single-cell RNA sequencing to clients worldwide, using advanced Drosophila microdissection and microfluidic techniques, as well as RNA sequencing knowledge. We help to study gene expression heterogeneity among individual cells and assist our clients in basic science and medical research.
What Can We Offer?
Drosophila cells are much smaller than mammalian cells, resulting in low cell capture efficiency and further reducing the absolute amount of individual cellular RNA. CD BioSciences has developed a robust Drosophila single-cell RNA sequencing platform based on Drosophila cell lines and live tissue cell isolation. In addition, standardized and strict quality control processes ensure correctness and reproducibility of experimental methods and results at each step.
- Single-Cell Capture
- ScRNA-Seq Library Preparation
- ScRNA-Seq and Data Analysis
CD BioSciences supports single cell suspension preparation, single cell sorting (fluorescence-activated cell sorting (FACS) and microfluidic platforms) and capture of live single cells with microscopic manipulation. Due to the complexity of Drosophila cell types and the difficulty of single-cell manipulation, we will test multiple dissociative sorting methods to ensure strategies that are less damaging and maintain high cell activity.
CD BioSciences optimizes cDNA amplicon size by enzymatic fragmentation and size selection, and produces cDNA and barcode libraries using UMI, Drop-seq and Smart-seq, etc. Our professional quality control for library quality and concentration determination.
The Smart-seq method allows full-length cDNA coverage, the ability to perform isoform analysis, and capture of large polyploid cells, aiding in the study of Drosophila tissue, but at a higher cost and reduced throughput compared to the use of UMI platform.
CD BioSciences sequencing using illumina. Our professional bioinformatics team pre-processes and customizes the analysis of sequenced data. We provide a total solution for custom data interpretation not only limited to cell clustering analysis, cell type identification, cell trajectory analysis, differential expression analysis, gene-set analysis, etc.
Why CD BioSciences?
- Rich experience in Drosophila single-cell RNA sequencing
- Professional Drosophila single-cell capture and scRNA-seq platforms
- Reliable sequencing data and high-quality interpretation reports
- Fast turnaround, cost-effective and transparent pricing
CD BioSciences is an advanced sequencing and big data analysis biotechnology company. We are technology-oriented and provide total solution for Drosophila single cell RNA sequencing. Our professional team provides 24 × 7 customer service to every client for saving time and effort. There are no limitations of our service. If you need any further information or have any question, please feel free to contact us.
- Hwang B, et al. (2018). Single-cell RNA sequencing technologies and bioinformatics pipelines. Exp Mol Med. 50, 1–14.
- Li H. (2021). Single‐cell RNA sequencing in Drosophila: Technologies and applications. Wiley Interdisciplinary Reviews: Developmental Biology. 10(5), e396.
For research use only. Not intended for any clinical use.