Recombinant D. melanogaster Protein PDF(Pdf)

Drosophila melanogaster

Pdf

Pdf; CG6496; Protein PDF [Cleaved into: PDF precursor-related peptide; PAP); Neuropeptide PDF; Pigment-dispersing factor homolog)]

25-79

Secreted.

Arthropod PDH family

Neuropeptide PDF is the main transmitter regulating circadian locomotor rhythms. Required to maintain behavioral rhythms under constant conditions by coordinating pacemaker interactions in the circadian system. Ectopic expression induces long periods, while its absence leads to short periods. Acts on small and large ventral lateral neurons to control sleep and regulates the state transition from sleep to wake.

KEGG: Dmel_CG6496; STRING: 7227.FBpp0084396; UniGene: Dm.21687

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MAMPDE ERYVRKEYNR DLLDWFNNVG VGQFSPGQVA TLCRYPLILE NSLGPSVPI

Cytoplasmic Domain

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.