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Drosophila Behavior Phenotypes Assay

Drosophila melanogaster is an advanced model organism, capable of both innate and complex behaviors. The behavior phenotype, although often overlooked, is an important indicator for detecting physiological impairments (e.g., feeding, locomotion) in animals. Moreover, as a neurologically studied organism, valid and objective phenotypes analysis of Drosophila can also assess changes in central regulatory functions.

CD BioSciences is committed to analyzing and quantifying Drosophila behavior phenotypes with data and images without bias, helping our clients' cutting-edge scientific research and saving time and effort. We customize multi-item, targeted behavioral tests and data collation for your project to ensure your follow-up experiments.

Fig.1 Behavioral components of courtship in Drosophila (Aranha et al, 2018)

What We Offer

As an expert in Drosophila research, CD BioSciences is equipped with a dedicated phenotypic assay laboratory and strictly control environmental factors such as temperature, humidity, light and food to reduce environmental effects on Drosophila behavior. We are equipped with a professional Drosophila activity monitoring system that supports all behavioral data recording of larvae and adults, as well as subsequent data collation and statistics (hypothesis testing, reliability analysis, correlation analysis, variance analysis, regression analysis, etc.).

If you would like to assay your own samples, please mail them to us 2 months prior to the start of the project, as we require parasite, virus, Wolbachia and viability testing prior to stocking.

Larval Phenotypes Assay Project

Larval detection is suitable for the death of pupal or adult by gene expression, and also can be used to detect the effect on the viability of Drosophila during the whole developmental stage.

Items	Descriptions
Larval Locomotion	The number of body wall contractions of early third instar larvae in a period of time. To examine the effects of drugs or disease genes on movement.
Larval Feeding	The number of the larval mouth hooks contractions of early third instar larvae. To examine the effects of chemicals, microbes, etc., or genetic mutations on feeding.
Larval Chemotaxis	Quantify the duration of running and direction of turning of the larvae. To study nerve regulation such as gustation and olfactory, or to evaluate the normality of directional movement.
Larval Phototaxis	Drosophila larvae are negative phototaxis during most of their development. To study the photosensitive mechanism and neural circuit of larvae.

Adult Phenotypes Assay Project

Items	Descriptions
Geotaxis	Flies has obvious geotaxis and can quickly and easily identify individuals with common physical defects
Loss of Righting Reflex	Flies have a rapid righting reflex. To study the damage of genetic disease and drug action to coordination.
Odor Response	Determine response to an attractive or repulsive odor. The neuroscientific research of smell, taste and chemical perception.
Response to the Sedative Effects of Ethanol	Fly is a very conservative response to alcohol. Usually for gender differences, neuroscience, circadian rhythm, depression and anxiety,etc.
Locomotion	Simple locomotion and exploratory behavior of flies is assessed by recording the behavior in a limited space for a short period of time.
Light–Dark Box	Record the number of transitions between dark and light surfaces (< 15 mins). To assess exploratory and adaptive behavior.
Forced Swim Test	The assay in flies is used to assess motivation for escape behavior. To study the genetic basis of stress-related mental illness.
Courtship	Indicators: orientation, wing extension, tapping, licking, curling. To investigate the genetic basis of sexual behavior, examine activity and coordination, and learning and memory.
Sleep	The number of flies passing through the infrared beam was monitored 24 hours, and the duration of sleep (>5 min), the number of sleep attacks, the average sleep attack time and waking activity were calculated. Applied to sleep and circadian rhythm research.
Aggressive Behavior	Kick leg extension, chase, wing-raise extension, box high impact interaction, etc. To study the genetic basis and neural regulation of aggressive behavior.
Forced Climbing Assay	To assess the locomotor capacity of fruit flies. Used in neuropathy research and easy for high throughput determination.
Gap-Crossing Assay	Using flies carrying blinding genes, record the ability to cross different gaps (1-4 mm). To study coordination and fine movement in fruit flies.

Why CD BioSciences?

Rich experience in designing behavior monitoring experiments
Excellent and professional research team and technical platform
Flexible Drosophila behavior assay and customized statistical analysis services
Reliable experimental data with good repeatability
Fast turnaround, cost-effective and transparent pricing

CD BioSciences is an expert in fruit fly field. We provide professional solutions for Drosophila behavior phenotypes. Our team of professionals provides 24 × 7 customer service to every client for saving time and effort. There are no limitations of our service. If you need any further information or have any question, please feel free to contact us.

Case Studies

Case 1

Aging Intervention Pipeline in Drosophila

Client: Aging research institute
Challenge: Screen natural compounds for anti-aging effects in a short time
Solution: Lifespan and climbing assays in Drosophila under compound treatment; data analyzed via automated imaging and scoring system
Outcome: Identified 3 candidates that extended median lifespan by >15%

Reference

Aranha MM, et al. (2018). Deciphering Drosophila female innate behaviors. Current opinion in neurobiology. 52, 139-148.

For research use only. Not intended for any clinical use.

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