Recombinant D. melanogaster Transformer-2 sex-determining protein(tra2)

Drosophila melanogaster

tra2

tra2; tra-2; CG10128; Transformer-2 sex-determining protein

1-264

Splicing factor SR family

Required for female sex determination in somatic cells and for spermatogenesis in male germ cells. Positive regulator of female-specific splicing and/or polyadenylation of doublesex (dsx) pre-mRNA. Splicing requires an enhancer complex, dsxRE (dsx repeat element: which contains six copies of a 13-nucleotide repeat and a purine-rich enhancer (PRE)). DsxRE is formed through cooperative interactions between tra, tra2 and the sr proteins, and these interactions require both the repeat sequences and PRE. PRE is required for specific binding of tra2 to the dsxRE. Protein-RNA and protein-protein interactions are involved in tra-2 dependent activation and repression of alternative splicing. Together with tra-2, plays a role in switching fru splicing from the male-specific pattern to the female-specific pattern through activation of the female-specific fru 5'-splice site.

KEGG: Dmel_CG10128; STRING: 7227.FBpp0088562; UniGene: Dm.4216

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MDREPLSSGR LHCSARYKHK RSASSSSAGT TSSGHKDRRS DYDYCGSRRH QRSSSRRRSR SRSSSESPPP EPRHRSGRSS RDRERMHKSR EHPQASRCIG VFGLNTNTSQ HKVRELFNKY GPIERIQMVI DAQTQRSRGF CFIYFEKLSD ARAAKDSCSG IEVDGRRIRV DFSITQRAHT PTPGVYLGRQ PRGKAPRSFS PRRGRRVYHD RSASPYDNYR DRYDYRNDRY DRNLRRSPSR NRYTRNRSYS RSRSPQLRRT SSRY

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.