Recombinant D. melanogaster Transcription initiation factor IIA subunit 2(TfIIA-S)

Drosophila melanogaster

TfIIA-S

TfIIA-S; CG5163; Transcription initiation factor IIA subunit 2; General transcription factor IIA subunit 2; TFIIA p14 subunit; TFIIA-14; Transcription initiation factor IIA gamma chain; TFIIA-gamma; dTFIIA-S

1-106

Nucleus.

TFIIA subunit 2 family

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Part of a rhi-dependent transcription machinery that enables the generation of piRNA precursors from heterochromatin while maintaining the suppression of transposon-encoded promoters and enhancers. Forms a complex with Moonshiner/CG12721 and Trf2 which recruit transcriptional machinery to heterochromatin to initiate the bidirectional transcription of piRNA clusters, by interacting with the RDC (rhi, del and cuff) complex that binds to repressive H3K9me3 marks in the chromatin. This mechanism allows transcription to occur in piRNA clusters despite the lack of proper promoter elements and in the presence of the repressive H3K9me3 mark.

KEGG: Dmel_CG5163; STRING: 7227.FBpp0083912; UniGene: Dm.20591

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MSYQLYRNTT LGNTLQESLD ELIQYGQITP GLAFKVLLQF DKSINNALNQ RVKARVTFKA GKLNTYRFCD NVWTLMLNDV EFREVHEIVK VDKVKIVACD GKSGEF

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.