Recombinant D. melanogaster RNA 3'-terminal phosphate cyclase

Species	Drosophila melanogaster
Target Names	CG4061
Alternative Names	CG4061; RNA 3'-terminal phosphate cyclase; RNA cyclase; RNA-3'-phosphate cyclase; EC 6.5.1.4
Expression Region	1-361
Subcellular Location	Nucleus, nucleoplasm.
Protein Families	RNA 3'-terminal cyclase family, Type 1 subfamily
Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
Database Info	KEGG: Dmel_CG4061; STRING: 7227.FBpp0070316; UniGene: Dm.33463

Product Type	Recombinant Protein
Source	Yeast; E. coli; Baculovirus; Mammalian cell
Target Protein Sequence	MDAEKMLEID GSYLEGGGQA LRNALSLSCI LGKPVRVVKI RASRPSPGLS HQHLHGLNLL RDITNADVVG NYLLSSTVEF TPRTILDNTY RVETHTAASI TLIYQMALPV LLFAGRPSRL IVSGGTNVDF APPVEYMQEV LLPNLKHFGV SFDLKVQRYG FYPRGQGRCQ LDVQPVTKLN SGKLVAFGRI KSVSGVAYCA GRLPVNIAID MQQTAQREIH RLWPSQQCSI EPIKHSRQKA FHNGAGILMT VNTTSDVVLG ASALGKKRID GHVLGSEASC QLGDYMRKQV CVDDYMQDQL IIYMALAVGR STMRTGKLTN HTRTAINVAE QMTGVKFDVA MEPGGQMLVS CKGLGHVNKL I
Protein Length	Full Length Protein
Purity	>85% (SDS-PAGE)
Tag Info	The following tags are available. N-terminal His-tagged Tag-Free
Form	Lyophilized powder
Buffer before Lyophilization	Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time	Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Storage Condition	Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.
Shelf Life	The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.
Handling	Repeated freezing and thawing are not recommended.
Limitations	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.

For research use only. Not intended for any clinical use.