Recombinant D. melanogaster Ras-like GTP-binding protein Rho1(Rho1)

Drosophila melanogaster

Rho1

Rho1; CG8416; Ras-like GTP-binding protein Rho1

1-189

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Apical cell membrane. Lateral cell membrane.

Small GTPase superfamily, Rho family

Has a role in regulating actin cytoskeletal organization: required during early development for proper execution of morphogenetic movements of individual cells and groups of cells important for the formation of the embryonic body plan. Plays a role in regulating dorsal closure during embryogenesis. During axis elongation, required for Rho-kinase Rok planar polarity and adherens junction localization as well as for generating a planar polarized distribution of the actin-binding protein Shrm. During embryogenesis, acts upstream of wash to regulate the developmental migration of tail hemocytes anteriorly along the ventral midline. May have a role in eye development.

KEGG: Dmel_CG8416; STRING: 7227.FBpp0086354; UniGene: Dm.644

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MTTIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRNDPNTIRD LAKMKQEPVK PQEGRAMAEK INAFAYLECS AKSKEGVRDV FETATRAALQ VKKRKKTRC

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.