Recombinant D. melanogaster Protein pelota(pelo)

Drosophila melanogaster

pelo

pelo; CG3959; Protein pelota; EC 3.1.-.-

1-395

Nucleus. Cytoplasm.

Eukaryotic release factor 1 family, Pelota subfamily

Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway. In the presence of pix and HSB1, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes. Together with HBS1 and in presence of pix, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway. Required prior to the first meiotic division for spindle formation and nuclear envelope breakdown during spermatogenesis. Together with HBS1, promotes spermatid individualization during spermatogenesis. Required for ovarian germ line stem cell self-renewal and oocyte development during oogenesis. Together with HSB1, required for transposon silencing in the ovary and testis. As part of the Pink1-regulated signaling, is recruited to damaged mitochondrial and is required for recruitment of autophagy receptors and induction of mitophagy. Required for normal eye patterning and for mitotic divisions in the ovary.

KEGG: Dmel_CG3959; STRING: 7227.FBpp0079444; UniGene: Dm.4742

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MKLLGKYVDK GMQGNVTLVP EESEDMWHAY NLIAKGDSVR STTIRKVQNE TATGSSTSSR VRTTLTIAVE SIDFDTQACV LRLKGRNIEE NQYVKMGAYH TLDLELNRKF ELRKPEWDTI ALERIEMACD PTQSADVAAV VMQEGLAHVC LITASMTLVR SKIEVSIPRK RKGSVQQHEK GLAKFYEQVM QSILRHVNFD VVKCVLIASP GFVRDQFYDY MFQQAVKMDY KLLLDNKSKF MLVHASSGFK HSLREILQDP AVLAKMSDTK AAGEVKALEQ FYMMLQCEPA KAFYGKKHVL QAAESQAIET LLISDNLFRC QDVSLRKEYV NLVESIRDAG GEVKIFSSMH ISGEQLAQLT GIAALLRFPM PELEDSDDDD DEDGAAGGVA DSDSD

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.