Recombinant D. melanogaster Partner of Y14 and mago(wibg)

Drosophila melanogaster

Pym

Pym; wibg; CG30176; Partner of Y14 and mago; DmPYM; Protein within the bgcn gene intron

1-207

Cytoplasm. Nucleus.

Pym family

Regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmark for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as an EJC disassembly factor by disrupting mature EJC from spliced mRNAs. Required for normal localization of osk mRNA to the posterior pole of the developing oocyte. Does not interact with the small ribosomal unit or components of the translation initiation complex. May not function in cap-dependent translation regulation.

KEGG: Dmel_CG30176; STRING: 7227.FBpp0072142; UniGene: Dm.12746

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MSTYLQSSEG KFIPATKRPD GTWRKARRVK DGYVPQEEVP LYESKGKQFV AQRQAGVPPG MCPLLAAESK KEREKQERTR AKKQEKESGR QPKAPAPGVL VMPPSTCPPP KVSQQQQQQQ QQPSGSRDIN SISKTLEDTL KLDAAQEVVD PAKQLKKLRK KIREIEQIES RIQAGEQKKL DKDQLDKVKK KSEILRQIKD LESTPRS

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.