Recombinant D. melanogaster Homeobox protein homothorax(hth)

Drosophila melanogaster

hth

hth; dtl; CG17117; Homeobox protein homothorax; Homeobox protein dorsotonals

1-487

Nucleus.

TALE/MEIS homeobox family

All isoforms are required for patterning of the embryonic cuticle. Acts with exd to delimit the eye field and prevent inappropriate eye development. Isoforms that carry the homeodomain are required for proper localization of chordotonal organs within the peripheral nervous system and antennal identity; required to activate antennal-specific genes, such as sal and to repress the leg-like expression of dac. Necessary for the nuclear localization of the essential HOX cofactor, extradenticle (exd). Both necessary and sufficient for inner photoreceptors to adopt the polarization-sensitive 'dorsal rim area' (DRA) of the eye fate instead of the color-sensitive default state. This occurs by increasing rhabdomere size and uncoupling R7-R8 communication to allow both cells to express the same opsin rather than different ones as required for color vision.

KEGG: Dmel_CG17117; STRING: 7227.FBpp0081732; UniGene: Dm.2272

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MAQPRYDDGL HGYGMDSGAA AAAMYDPHAG HRPPGLQGLP SHHSPHMTHA AAAAATVGMH GYHSGAGGHG TPSHVSPVGN HLMGAIPEVH KRDKDAIYEH PLFPLLALIF EKCELATCTP REPGVQGGDV CSSESFNEDI AMFSKQIRSQ KPYYTADPEV DSLMVQAIQV LRFHLLELEK VHELCDNFCH RYISCLKGKM PIDLVIDERD TTKPPELGSA NGEGRSNADS TSHTDGASTP DVRPPSSSLS YGGAMNDDAR SPGAGSTPGP LSQQPPALDT SDPDGKFLSS LNPSELTYDG RWCRREWSSP ADARNADASR RLYSSVFLGS PDNFGTSASG DASNASIGSG EGTGEEDDDA SGKKNQKKRG IFPKVATNIL RAWLFQHLTH PYPSEDQKKQ LAQDTGLTIL QVNNWFINAR RRIVQPMIDQ SNRAVYTPHP GPSGYGHDAM GYMMDSQAHM MHRPPGDPGF HQGYPHYPPA EYYGQHL

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.