Recombinant D. melanogaster Elongation factor 1-alpha 2(Ef1alpha100E)

Species	Drosophila melanogaster
Target Names	Ef1alpha100E
Alternative Names	eEF1alpha2; EF1a; Ef1alpha100E; F2; CG1873; Elongation factor 1-alpha 2; EF-1-alpha-2
Expression Region	1-462
Subcellular Location	Cytoplasm.
Protein Families	TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-Tu/EF-1A subfamily
Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Database Info	KEGG: Dmel_CG1873; STRING: 7227.FBpp0085193; UniGene: Dm.1894

Product Type	Recombinant Protein
Source	Yeast; E. coli; Baculovirus; Mammalian cell
Target Protein Sequence	MGKEKIHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAQEMG KGSFKYAWVL DKLKAERERG ITIDIALWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGT GEFEAGISKN GQTREHALLA FTLGVKQLIV GVNKMDSTEP PYSEARYEEI KKEVSSYIKK IGYNPASVAF VPISGWHGDN MLEPSEKMPW FKGWSVERKE GKAEGKCLID ALDAILPPQR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG LLKPGMVVNF APVNLVTEVK SVEMHHEALT EAMPGDNVGF NVKNVSVKEL RRGYVAGDSK NNPPRGAADF TAQVIVLNHP GQIANGYTPV LDCHTAHIAC KFSEIKEKCD RRTGKTTETE PKAIKSGDAA IIVLVPSKPL CVESFQEFPP LGRFAVRDMR QTVAVGVIKS VNFKETTSGK VTKAAEKAQK KK
Protein Length	Full Length Protein
Purity	>85% (SDS-PAGE)
Tag Info	The following tags are available. N-terminal His-tagged Tag-Free
Form	Lyophilized powder
Buffer before Lyophilization	Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time	Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Storage Condition	Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.
Shelf Life	The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.
Handling	Repeated freezing and thawing are not recommended.
Limitations	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.

For research use only. Not intended for any clinical use.