Recombinant D. melanogaster 3-hydroxyacyl-CoA dehydrogenase type-2(scu)

Drosophila melanogaster

scu

scu; CG7113; 3-hydroxyacyl-CoA dehydrogenase type-2; EC 1.1.1.35; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; EC 1.1.1.51; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; EC 1.1.1.178; 3-hydroxyacyl-CoA dehydrogenase type II; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Scully protein; Type II HADH

1-255

Mitochondrion.

Short-chain dehydrogenases/reductases (SDR) family

Versatile enzyme presenting two types of activity; L-3-hydroxyacyl-CoA dehydrogenase ((3S)-3-hydroxyacyl-CoA dehydrogenase) activity and hydroxysteroid dehydrogenase (HSD) activity with a wide substrate spectrum. As a (3S)-3-hydroxyacyl-CoA dehydrogenase, it functions in the third step of the fatty acid beta-oxidation pathway, a major metabolic process in which fatty acids are oxidized to provide a significant source of energy, while also generating acyl-CoA metabolites used by many metabolic routes (Probable). As a HSD, it functions in the degradation pathways of glucocorticoids and sex steroids and epimerization of bile acids; catalyzes the beta-oxidation at position 17 of androgens and estrogens, has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone, and carries out oxidative conversions of 7-beta-hydroxylated bile acids like ursodeoxycholate or isoursodeoxycholate (also known as 3-beta,7-beta-dihydroxy-5-beta-cholan-24-oate or 7-beta-hydroxyisolithocholate, respectively). Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. Required for cell survival during embryonic development. May play a role in germline formation.

KEGG: Dmel_CG7113; STRING: 7227.FBpp0074285; UniGene: Dm.7957

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MIKNAVSLVT GGASGLGRAT AERLAKQGAS VILADLPSSK GNEVAKELGD KVVFVPVDVT SEKDVSAALQ TAKDKFGRLD LTVNCAGTAT AVKTFNFNKN VAHRLEDFQR VININTVGTF NVIRLSAGLM GANEPNQDGQ RGVIVNTASV AAFDGQIGQA AYSASKAAVV GMTLPIARDL STQGIRICTI APGLFNTPML AALPEKVRTF LAKSIPFPQR LGEPSEYAHL VQAIYENPLL NGEVIRIDGA LRMMP

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.