Recombinant D. melanogaster 14-3-3 protein epsilon(14-3-3epsilon)

Species	Drosophila melanogaster
Target Names	14-3-3epsilon
Alternative Names	14-3-3epsilon; 14-3-3e; SR3-9; CG3119614-3-3 protein epsilon; Suppressor of Ras1 3-9
Expression Region	1-262
Protein Families	14-3-3 family
Function	Positively regulates Ras-mediated pathways. Acts downstream or parallel to Raf, but upstream of nuclear factors in Ras signaling. Three mutants have been isolated, that suppress the rough eye phenotype caused by mutated Ras1 (sev-Ras1 v12). Inhibits yki activity by restricting its nuclear localization. Together with pav, has a role in the inhibition of microtubule sliding during neurite outgrowth.
Database Info	KEGG: Dmel_CG31196; STRING: 7227.FBpp0082987; UniGene: Dm.2357

Product Type	Recombinant Protein
Source	Yeast; E. coli; Baculovirus; Mammalian cell
Target Protein Sequence	MTERENNVYK AKLAEQAERY DEMVEAMKKV ASMDVELTVE ERNLLSVAYK NVIGARRASW RIITSIEQKE ENKGAEEKLE MIKTYRGQVE KELRDICSDI LNVLEKHLIP CATSGESKVF YYKMKGDYHR YLAEFATGSD RKDAAENSLI AYKAASDIAM NDLPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQAEEV DPNAGDGEPK EQIQDVEDQD VS
Protein Length	Full Length Protein
Purity	>85% (SDS-PAGE)
Tag Info	The following tags are available. N-terminal His-tagged Tag-Free
Form	Lyophilized powder
Buffer before Lyophilization	Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time	Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Storage Condition	Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.
Shelf Life	The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.
Handling	Repeated freezing and thawing are not recommended.
Limitations	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.

For research use only. Not intended for any clinical use.